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KMID : 0358920050320040662
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Journal of the Korean Academy of Pedodontics
2005 Volume.32 No. 4 p.662 ~ p.669
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EXPRESSION OF DSPP AND BSP MRNAS DURING ODONTOBLAST DIFFERENTIATION IN THE NFI-C(-/-)MICE
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Jung Hyun-Ku
Park Ju-Cheol
Lee Sang-Ho
Lee Chang-Seep
Lee Nan-Young
Kim Heung-Joong
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Abstract
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Nuclear factor I (NFI) exists in the odontoblast and osteoblast.NFI-C null mice demonstrated aberrant odontoblast differentiation, abnormal dentin formation, and molar lacking roots.
The purpose of this study was to examine phenotype of the aberrant odontoblast in NFI-C null mice and to evaluate the expression of DSPP and BSP mRNAs in NFI-C null mice with in-situ hybridization.
The results were as follows:
1.In the NFI-C (-/-) mice, the crown dentin of molar showed normally formation, but there was no root dentin.
2.In the NFI-C (-/-) mice, the labial dentin of mandibular incisors showed relatively a lot of dentin formation, but the lingual dentin showed defect.
3.In the NFI-C (-/-) mice, the odontoblast of mandibular incisors revealed abnormal shape and trapped in osteodentin-like mineralized tissue.
4.In the NFI-C (-/-) mice, the odontoblast in the crown dentin of molars showed strong expression of DSPP, the odontoblast in the root dentin of molars was not expression of DSPP. In the NFI-C (-/-) mice the odontoblast in the mandibular incisors showed weekly expression of DSPP.
5.In the wild mice, the odontoblasts of mandibular incisors were not expression of BSP, but in the NFI-C (-/-) mice the odontoblast of mandibular incisors showed strong expression of BSP.
These results suggest that odontoblst in the NFI-C (-/-) mice changes the phenotype into osteoblast.
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KEYWORD
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NFI-C, Odontoblast differentiation, DSPP, BSP
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